Roy et al.: Computer-assisted simulations...

Full text PDF files should be viewed and printed using Adobe^® Acrobat^® Reader version 3.01 or higher (Download here). Further details about PDF and Adobe^® Acrobat^® Reader can be found on our "PDF FAQ" page.

Biochemistry and Molecular Biology Education, Vol. 29 (1) (2001) pp. 3-9
© 2001 IUBMB. Published by Elsevier Science Ltd. All rights reserved.
PII: S1470-8175(00)00059-X

Article

Computer-assisted simulations of phosphofructokinase-1 kinetics using simplified velocity equations

Harry Roy ^a royh@rpi.edu, Joyce Diwan ^a, Leigh D. Segel ^b and Irwin H. Segel ^b ^* ihsegel@ucdavis.edu

^a Department of Biology, MRC302, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA
^b Section of Molecular and Cellular Biology, University of California, One Shields Avenue, Davis, CA 95616, USA

Abstract

Equations useful for simulating the kinetic behavior of phosphofructokinase are presented. The equations, which are based on the concerted transition (symmetry) model for allosteric enzymes, account for substrate inhibition by MgATP, cooperative binding by F-6-P, activation by F-2,6-P₂, and deinhibition by AMP. Velocity calculations can be performed using either a spreadsheet program (e.g., MS Excel) or a web-based program (e.g., Authorware). Both approaches are illustrated.

Keywords: Phosphofructokinase; Simulations; Allosteric; Computer; Cooperativity; Kinetics

^*Corresponding author. Tel: 530-752-3193; fax: 530-752-3085

[Full text] (PDF 644.1 Kb)

Article

Computer-assisted simulations of phosphofructokinase-1 kinetics using simplified velocity equations

Harry Roy a royh@rpi.edu, Joyce Diwan a, Leigh D. Segel b and Irwin H. Segel b * ihsegel@ucdavis.edu

Abstract

Harry Roy ^a royh@rpi.edu, Joyce Diwan ^a, Leigh D. Segel ^b and Irwin H. Segel ^b ^* ihsegel@ucdavis.edu