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Biochemistry and Molecular Biology Education, Vol. 29 (2) (2001) pp. 71-75
© 2001 IUBMB. Published by Elsevier Science Ltd. All rights reserved.
PII: S1470-8175(01)00016-9

Laboratory Exercises

Analysis of the tetranucleotide polymorphic HUMTH01 microsatellite

Isabelle Lajoie-Mazenc * lajoie@icr.fnclcc.fr and Bettina Couderc Couderc_b@icr.fnclcc.fr

UPRES EA 2048, Institut Claudius Regaud and Faculté de Pharmacie, 20-24 rue du pont St Pierre, 31052 Toulouse, France

Abstract

The major aim of this practical class in molecular biology is to familiarize students with some common manipulations of DNA (extraction from buccal swabs, electrophoresis, etc.) and to allow them to carry out the polymerase chain reaction, on their own DNA, in order to amplify a sequence containing a tetranucleotide microsatellite (HUMTH01). This class is also providing experience in DNA finger printing by comparing the size of the sequences amplified from each student's DNA. These sequences correspond to tetranucleotide microsatellite polymorphisms.

Keywords: Microsatellite analysis; Polymorphism; PCR; DNA electrophoresis

*Corresponding author. Tel.: +33-5-61-42-42-23; fax : +33-5-61-42-46-31

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