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Biochemistry and Molecular Biology Education, Vol. 29 (5) (2001) pp. 196-198
© 2001 Published by Elsevier Science Ltd. All rights reserved.
PII: S1470-8175(01)00074-1

Laboratory exercises

Aminotransferase from insect cells

Hilary Erwin, Grace de Ridder and Peter A Barton * pbarton@swin.edu.au

School of Engineering and Science, Swinburne University of Technology, Hawthorn, Vic., 3122 Australia

Abstract

The presence of transaminase activity is demonstrated in the cultured insect cell line Sf21 (Spodoptera frugiperda) at a level sufficient to enable replacement of rat liver in an undergraduate student experiment designed to demonstrate enzyme activity responsible for amino acid interconversions.

Keywords: Alanine:2-oxoglutarate aminotranferase (EC. 2.6.1.2); Alanine aminotransferase; Transaminase; Insect; Spodoptera frugiperda; Sf21

*Corresponding author

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