| Full text PDF files should be viewed and printed using Adobe® Acrobat® Reader version 3.01 or higher (Download here). Further details about PDF and Adobe® Acrobat® Reader can be found on our "PDF FAQ" page. |
Comparative cellular metabolism can be a difficult area of biochemistry to teach in the undergraduate laboratory class. Student practicals involving animal tissues generally require approval from animal ethic committees, and the relevance for students whose primary interest in biochemistry is in the area of food and wine sciences, is often questioned. In this report, we present an undergraduate practical exercise in which glucose catabolism via the pentose phosphate pathway is compared in two types of yeast with direct relevance to the wine and food industries, Saccharomyces cerevisiae and Kloeckera apiculata. The exercise is carried out as a demonstration to second year undergraduate students, studying metabolic biochemistry. It is of some value in that it illustrates comparative cellular metabolism in wine yeasts and introduces the students to the safe use of radioisotopes.
Keywords: Pentose phosphate pathway; Wine yeasts; Saccharomyces cerevisiae; Kloeckera apiculata; Hanseniaspora uvarum; [1-14C]-glucose; Carbohydrate metabolism*Corresponding author
1Current address: Department of Horticulture, Viticulture and Oenology, The University of Adelaide, Private Mail Bag 1, Glen Osmond, SA 5064, Australia.
[Full text] (PDF 129.7 Kb)
© Copyright 2008, Elsevier Science, All rights reserved.