Volume 1. Structure and Dynamics of Membranes

Chapter 1. Biological membranes architecture and function

E. Sackmann
Technische Universität München,
James-Franck-Strasse, D-85748 Garching, Germany

1. Introduction

1.1. Why are biomembranes a playground for physicists?
1.2. Biomembranes enable the modular design of cells
2. Molecular architecture of biomembranes
2.1. Cell plasma membrane as three layered composite system with associated macromolecular network
2.2. Erythrocyte birth and membrane structure
3. Molecular composition of the lipid/protein bilayer
3.1. Universalities and pecularities of lipid composition
3.2. Membranes of cellular organelles exhibit characteristic distributions (patterns) of lipids
3.3. The four major subclasses of membrane proteins
3.4. Bilayer asymmetry is an essential feature of the modular design of cells
4. Regulation of osmotic equilibrium: an ubiquitous task of membranes
4.1. Ion and molecular transport is controlled by only a few classes of pumps
4.2. P-type ATPase is a prototype of a functional protein which is powered by ATP-cleavage and driven by a conformational change following phosphorylation
4.3. Energetics and reversibility of pumps
4.4. The regulation of Na+ /K+ -ATPase activity by steroids as a prototype of drug action
4.5. Differentiation is often controlled by type and number of membrane associated pumps
4.6. The concerted action of many pumps holds electrochemical equilibrium and may control cell shape
5. Membrane synthesis, differentiation and recycling
5.1. Lipids are synthesized in the ER, the Golgi and the Mitochondria
5.2. Membrane and secretory proteins are synthesized by the ER and modified in the Golgi: afirststepsorting
5.3. Nascent proteins must be processed in various ways before their translocation to target spaces and membranes
5.4. Part of newly synthesized lipid and protein molecules are distributed on a molecular basis
6. Vectorial organisation of cells and material flow during metabolism and secretion
6.1. Synopsis
6.2. Phenomenology of trafficking from the ER to the plasma membrane
6.3. On the generation of lysosomes and lysosomal enzymes
6.4. Receptor mediated endocytosis of metabolites via coated pits: an example of vesicle and receptor recycling
6.5. The role of molecular filters for the directed material exchange and sorting
7. On global shape instabilities of cells
7.1. Phagocytosis
7.2. On the export of cellular organelles by budding and secretion
8. On the interplay between fast randomization and local phase separation in biomembranes
8.1. Diseases are often associated with changes in lipid composition
8.2. Two-dimensionality of membranes is essential for diffusion controlled processes and enables manipulation of lateral protein mobility over orders of magnitude
8.3. Hormone signal transduction: a possible example of the interplay between rapid lateral randomization and local aggregation
Abbreviations

References